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http://223.31.159.10:8080/jspui/handle/123456789/977
Title: | CRISPR-Cas9 system: A new-fangled dawn in gene editing |
Authors: | Gupta, Darshana Bhattacharjee, Oindrila Mandal, Drishti Sen, Madhab Kumar Dey, Dhritiman Dasgupta, Adhiraj Kazi, Tawsif Ahmed Gupta, Rahul Sinharoy, Senjuti Acharya, Krishnendu Chattopadhyay, Dhrubajyoti Ravichandiran, V. Roy, Syamal Ghosh, Dipanjan |
Keywords: | CRISPR-Cas9 Genome editing Knock out Knock in |
Issue Date: | 2019 |
Publisher: | Elsevier B.V. |
Citation: | Life Sciences, 232: 116636 |
Abstract: | Till date, only three techniques namely Zinc Finger Nuclease (ZFN), Transcription-Activator Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-Associated 9 (CRISPR-Cas9) are available for targeted genome editing. CRISPR-Cas system is very efficient, fast, easy and cheap technique for achieving knock-out gene in the cell. CRISPR-Cas9 system refurbishes the targeted genome editing approach into a more expedient and competent way, thus facilitating proficient genome editing through embattled double-strand breaks in approximately any organism and cell type. The off-target effects of CRISPR Cas system has been circumnavigated by using paired nickases. Moreover, CRISPR-Cas9 has been used effectively for numerous purposes, like knock-out of a gene, regulation of endogenous gene expression, live-cell labelling of chromosomal loci, edition of single-stranded RNA and high-throughput gene screening. The execution of the CRISPR-Cas9 system has amplified the number of accessible scientific substitutes for studying gene function, thus enabling generation of CRISPR-based disease models. Even though many mechanistic questions are left behind to be answered and the system is not yet fool-proof i.e., a number of challenges are yet to be addressed, the employment of CRISPR-Cas9–based genome engineering technologies will increase our understanding to disease processes and their treatment in the near future. In this review we have discussed the history of CRISPR-Cas9, its mechanism for genome editing and its application in animal, plant and protozoan parasites. Additionally, the pros and cons of CRISPR-Cas9 and its potential in therapeutic application have also been detailed here. |
Description: | Accepted date: 5 July 2019 |
URI: | http://223.31.159.10:8080/jspui/handle/123456789/977 |
ISSN: | 0024-3205 |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
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Sinharoy S_2019_3.pdf Restricted Access | 1.89 MB | Adobe PDF | View/Open Request a copy |
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