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DC Field | Value | Language |
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dc.contributor.author | Gupta, Darshana | - |
dc.contributor.author | Bhattacharjee, Oindrila | - |
dc.contributor.author | Mandal, Drishti | - |
dc.contributor.author | Sen, Madhab Kumar | - |
dc.contributor.author | Dey, Dhritiman | - |
dc.contributor.author | Dasgupta, Adhiraj | - |
dc.contributor.author | Kazi, Tawsif Ahmed | - |
dc.contributor.author | Gupta, Rahul | - |
dc.contributor.author | Sinharoy, Senjuti | - |
dc.contributor.author | Acharya, Krishnendu | - |
dc.contributor.author | Chattopadhyay, Dhrubajyoti | - |
dc.contributor.author | Ravichandiran, V. | - |
dc.contributor.author | Roy, Syamal | - |
dc.contributor.author | Ghosh, Dipanjan | - |
dc.date.accessioned | 2019-09-05T11:42:57Z | - |
dc.date.available | 2019-09-05T11:42:57Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Life Sciences, 232: 116636 | en_US |
dc.identifier.issn | 0024-3205 | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/977 | - |
dc.description | Accepted date: 5 July 2019 | en_US |
dc.description.abstract | Till date, only three techniques namely Zinc Finger Nuclease (ZFN), Transcription-Activator Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-Associated 9 (CRISPR-Cas9) are available for targeted genome editing. CRISPR-Cas system is very efficient, fast, easy and cheap technique for achieving knock-out gene in the cell. CRISPR-Cas9 system refurbishes the targeted genome editing approach into a more expedient and competent way, thus facilitating proficient genome editing through embattled double-strand breaks in approximately any organism and cell type. The off-target effects of CRISPR Cas system has been circumnavigated by using paired nickases. Moreover, CRISPR-Cas9 has been used effectively for numerous purposes, like knock-out of a gene, regulation of endogenous gene expression, live-cell labelling of chromosomal loci, edition of single-stranded RNA and high-throughput gene screening. The execution of the CRISPR-Cas9 system has amplified the number of accessible scientific substitutes for studying gene function, thus enabling generation of CRISPR-based disease models. Even though many mechanistic questions are left behind to be answered and the system is not yet fool-proof i.e., a number of challenges are yet to be addressed, the employment of CRISPR-Cas9–based genome engineering technologies will increase our understanding to disease processes and their treatment in the near future. In this review we have discussed the history of CRISPR-Cas9, its mechanism for genome editing and its application in animal, plant and protozoan parasites. Additionally, the pros and cons of CRISPR-Cas9 and its potential in therapeutic application have also been detailed here. | en_US |
dc.description.sponsorship | Authors acknowledge the funding agencies: DBT, Govt of India (Grant No - BT/PR 26301/GET/119/258/2017) and WB-DBT (63 (Sanc.)-BT/P/Budget/RD-74/2017). S. Roy is supported by JC Bose Fellowship SB/S2/JCB-65/2014. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier B.V. | en_US |
dc.subject | CRISPR-Cas9 | en_US |
dc.subject | Genome editing | en_US |
dc.subject | Knock out | en_US |
dc.subject | Knock in | en_US |
dc.title | CRISPR-Cas9 system: A new-fangled dawn in gene editing | en_US |
dc.type | Article | en_US |
dc.identifier.officialurl | https://www.sciencedirect.com/science/article/pii/S0024320519305624?via%3Dihub | en_US |
dc.identifier.doi | https://doi.org/10.1016/j.lfs.2019.116636 | en_US |
Appears in Collections: | Institutional Publications |
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File | Description | Size | Format | |
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Sinharoy S_2019_3.pdf Restricted Access | 1.89 MB | Adobe PDF | View/Open Request a copy |
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