Please use this identifier to cite or link to this item: http://223.31.159.10:8080/jspui/handle/123456789/97
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dc.contributor.authorPanjabi, Priya-
dc.contributor.authorJagannath, Arun-
dc.contributor.authorBisht, Naveen C.-
dc.contributor.authorPadmaja, K Lakshmi-
dc.contributor.authorSharma, Sarita-
dc.contributor.authorGupta, Vibha-
dc.contributor.authorPradhan, Akshay K-
dc.contributor.authorPental, Deepak-
dc.date.accessioned2013-11-11T07:07:25Z-
dc.date.available2013-11-11T07:07:25Z-
dc.date.issued2008-
dc.identifier.citationBMC Genomics, 9: 113en_US
dc.identifier.urihttp://hdl.handle.net/123456789/97-
dc.description.abstractBackground: Extensive mapping efforts are currently underway for the establishment of comparative genomics between the model plant, Arabidopsis thaliana and various Brassica species. Most of these studies have deployed RFLP markers, the use of which is a laborious and time-consuming process. We therefore tested the efficacy of PCR-based Intron Polymorphism (IP) markers to analyze genome-wide synteny between the oilseed crop, Brassica juncea (AABB genome) and A. thaliana and analyzed the arrangement of 24 (previously described) genomic block segments in the A, B and C Brassica genomes to study the evolutionary events contributing to karyotype variations in the three diploid Brassica genomes. Results: IP markers were highly efficient and generated easily discernable polymorphisms on agarose gels. Comparative analysis of the segmental organization of the A and B genomes of B. juncea (present study) with the A and B genomes of B. napus and B. nigra respectively (described earlier), revealed a high degree of colinearity suggesting minimal macro-level changes after polyploidization. The ancestral block arrangements that remained unaltered during evolution and the karyotype rearrangements that originated in the Oleracea lineage after its divergence from Rapa lineage were identified. Genomic rearrangements leading to the gain or loss of one chromosome each between the A-B and A-C lineages were deciphered. Complete homoeology in terms of block organization was found between three linkage groups (LG) each for the A-B and A-C genomes. Based on the homoeology shared between the A, B and C genomes, a new nomenclature for the B genome LGs was assigned to establish uniformity in the international Brassica LG nomenclature code. Conclusion: IP markers were highly effective in generating comparative relationships between Arabidopsis and various Brassica species. Comparative genomics between the three Brassica lineages established the major rearrangements, translocations and fusions pivotal to karyotype diversification between the A, B and C genomes of Brassica species. The inter-relationships established between the Brassica lineages vis-à-vis Arabidopsis would facilitate the identification and isolation of candidate genes contributing to traits of agronomic value in crop Brassicas and the development of unified tools for Brassica genomics.en_US
dc.description.sponsorshipThe work was financially supported by Dhara Vegetable Oil and Food Company Ltd (DOFCO), a subsidiary of the National Dairy Development Board (NDDB), India. Partial support came from the UGC-SAP programme. SS was funded by a fellowship from the Department of Science and Technology (DST) under the SERC Fast Track Scheme for Young Scientists. LP was supported by a research fellowship from the Council of Scientific and Industrial Research (CSIR).en_US
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.subjectBrassica genomesen_US
dc.subjectBrassica junceaen_US
dc.subjectArabidopsis thalianaen_US
dc.subjectIntron Polymorphismen_US
dc.subjectmappingen_US
dc.subjectmarkersen_US
dc.titleComparative mapping of Brassica juncea and Arabidopsis thaliana using intron polymorphism (IP) markers: homeologous relationships, diversification and evolution of the A, B and C Brassica genomesen_US
dc.typeArticleen_US
dc.date.AcceptedDate3 March 2008en_US
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