Please use this identifier to cite or link to this item:
http://223.31.159.10:8080/jspui/handle/123456789/981
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Verma, Subodh | - |
dc.contributor.author | Bhatia, Sabhyata | - |
dc.date.accessioned | 2019-09-11T07:21:41Z | - |
dc.date.available | 2019-09-11T07:21:41Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | 3 Biotech 9(9): 346 | en_US |
dc.identifier.issn | 2190-5738 | - |
dc.identifier.uri | http://223.31.159.10:8080/jspui/handle/123456789/981 | - |
dc.description | Accepted date: 14 August 2019 | en_US |
dc.description.abstract | The aim of this study was to provide a comprehensive analysis of the plant-specifc B3 domain-containing transcription factors (TFs) in chickpea. Scanning of the chickpea genome resulted in the identifcation of 51 B3 domain-containing TFs that were located on seven out of eight chickpea chromosomes. Based on the presence of additional domains other than the B3 domain, the candidates were classifed into four subfamilies, i.e., ARF (24), REM (19), LAV (6) and RAV (2). Phylogenetic analysis classifed them into four groups in which members of the same group had similar intron–exon organization and motif composition. Genome duplication analysis of the candidate B3 genes revealed an event of segmental duplication that was instrumental in the expansion of the B3 gene family. Ka/Ks analysis showed that the B3 gene family was under purifying selection. Further, chickpea B3 genes showed maximum orthology with Medicago followed by soybean and Arabidopsis. Promoter analyses of the B3 genes led to the identifcation of several tissue-specifc and stress-responsive cis-regulatory elements. Expression profling of the candidate B3 genes using publicly available RNA-seq data of several chickpea tissues indicated their putative role in plant development and abiotic stress response. These fndings were further validated by realtime expression analysis. Overall, this study provides a comprehensive analysis of the B3 domain-containing proteins in chickpea that would aid in devising strategies for crop manipulation in chickpea. | en_US |
dc.description.sponsorship | This work was funded by the Department of Biotechnology, Ministry of Science and Technology, Government of India, under the Challenge Programme on Chickpea Functional Genomics (Grant number: BT/AGR/CG-Phase II/01/2014). SV acknowledges the award of a research fellowship from the Department of Biotechnology, Govt. of India. The authors are thankful to DBT-eLibrary Consortium (DeLCON) for providing access to e-resources. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Springer Nature Publishing AG | en_US |
dc.subject | Chickpea | en_US |
dc.subject | B3 domain-containing protein | en_US |
dc.subject | Phylogeny | en_US |
dc.subject | Expression analysis | en_US |
dc.title | A comprehensive analysis of the B3 superfamily identifies tissue-specific and stress-responsive genes in chickpea (Cicer arietinum L.) | en_US |
dc.type | Article | en_US |
dc.identifier.officialurl | https://link.springer.com/article/10.1007%2Fs13205-019-1875-5 | en_US |
dc.identifier.doi | https://doi.org/10.1007/s13205-019-1875-5 | en_US |
Appears in Collections: | Institutional Publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Bhatia S_2019_1.pdf Restricted Access | 2.58 MB | Adobe PDF | View/Open Request a copy |
Items in IR@NIPGR are protected by copyright, with all rights reserved, unless otherwise indicated.